IMMUNOLOGICAL BIOTECHNOLOGY

- Classic Vaccinology
- Main problems in the development of a vaccine.
- production of recombinant vaccines
- Microbial, animal and plant models for vaccine production.
- Reverse vaccinology: genome based antigen individuation (in silico). Production, quality control.
Main vaccines in pediatric prevention in Italy.
Adjuvants - Mucosal adjuvants - micro-nanosized new generation adjuvants.
- Use of dendritic cells in therapy: perspectives.

Practical part:

Evaluation in vitro of adjuvancy in human dendritic cells. Isolation of monocytes fromm blood, thier differentiation into Dendritic Cells (DCs). Stimulation of DCs with various adjuvants and analysis of cell activation by Elisa (TNFa) and flow cytometry (CD86, CD11), RT-PCR (tnfa gene transcription). Aultologous/heterologous T lymphocytes proliferation and characterisation of their immunological competence by FACS.