NANOBIOTECHNOLOGY (RAFFAELLA SIGNORINI)

Unit (i) Physical Chemistry of the Nanosystems:
Physical and chemical properties of bulk and nanomaterials: highlights on the electronic structure of atoms, molecules, metallic, semiconductors and insulating materials. Intermolecular forces and molecular recognition, surface interactions, molecular self-assembly. Structure and properties of metal, semiconductor and oxide nanoparticles.
Highlights on the techniques used to characterize nanomaterials:
X-ray diffraction, Optical and electronic microscopy, Scanning Probe Microscopy: STM and AFM, Absorption and emission spectroscopy, IR and Raman spectroscopy, Micro-raman and SERS.
Highlights on the use of nanoparticles and macromolecular compounds in the bio-medical field:


Unit (ii) Bio-Imaging

Electromagnetic waves
Refractive index
Propagation laws
Optical systems and image formation
Fluorescence: molecular apspects
Conventional fluorescence microscope
Point Spread Function (PSF) and resolving power of the microscope
Confocal Microscopy
Multiphoton Microscopy
STED Microscopy
Ca2+ Imaging
FRET
FRAP