PROTEIN ENGINEERING
Corso
A Padova
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Descrizione
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Tipologia
Corso
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Luogo
Padova
Evaluation will be subdivided in three parts:
1) Bibliographic search and oral presentation of the results. Each student will perform a bibliographic search regarding a particular aspect of a topic dealt with in classes.
2) Written report on the practical part of the course
3) Written examination regarding the course topics
Sedi e date
Luogo
Inizio del corso
Inizio del corso
Opinioni
Programma
The topics covered are as follows:
- protein stability and strategies to improve stability
-methods of directed mutagenesis of proteins
-methods of random mutagenesis of proteins
-methodologies to create libraries of mutants (directed evolution) and methods of high-throughput screening
-methods for the production and modification of antibodies, antibody fragments and new binding proteins developed from non-immunoglobulin domains
-circular permutation of proteins
-inteins and their applications
-production of proteins and peptides containing non-natural amino acids.
Example of application in research and in biotechnology industry will be provided in order to better illustrate these techniques.
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PROTEIN ENGINEERING