PROTEIN STRUCTURE AND DYNAMICS
Corso
A Padova
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Descrizione
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Tipologia
Corso
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Luogo
Padova
Oral questions with the option to define with the lecturer a specific topic or a case study to discuss at the beginning of the exam.
Sedi e date
Luogo
Inizio del corso
Inizio del corso
Opinioni
Programma
2. Relaxation, dipolar coupling, nuclear Overhauser effect (NOE).
3. Practical aspects in the acquisition of NMR spectra: principal components of NMR spectrometer, acquisition and processing of NMR signals, preparation of samples suitable for the NMR analysis.
4. Principle of FT-NMR; Producat Operator formalism to describe NMR experiments.
3. Homonuclear 2D NMR experiments (COSY, TOCSY, NOESY).
6. Heteronuclear correlation spectroscopy: INEPT, HSQC and HMQC experiment.
7. Triple resonance NMR experiments for the study of proteins.
8. Use of NMR data for the structural characterization of peptides and proteins: secondary structure from NMR parameters, high resolution tertiary fold from NOE data. Structure validation.
9. Spin relaxation measurments to evaluate protein dynamics
10.Strategies to study large proteins: TROSY experiments and deuteration.
11. Residual Dipolar Couplings in structural biology.
12. Protein-and protein-ligands interactions by NMR.
13. Production of recombinant labeled proteins.
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PROTEIN STRUCTURE AND DYNAMICS